![]() Their placement in the genus Brucella has been supported by the following characteristics: they are aerobic, non-motile and catalase-positive, do not produce acid from carbohydrates and have a DNA-DNA relatedness value of >77% with the six established members of the genus. Small Gram-negative cocco-bacilli resembling Brucella strains have been reported from marine mammals since the mid-1990s. Because of its unexpected isolation, the name Brucella inopinata with the type strain BO1(T) (=BCCN 09-01(T)=CPAM 6436(T)) is proposed. From the phenotypic and molecular analysis it became evident that strain BO1( T) was clearly different from all other Brucella species, and therefore represents a novel species within the genus Brucella. Using the Brucella species-specific multiplex PCR assay, strain BO1(T) displayed a unique banding pattern not observed in other Brucella species. The recA gene sequence of strain BO1(T) differed in seven nucleotides from the Brucella recA consensus sequence. Comparative omp2a and omp2b gene sequence analysis revealed the most divergent omp2 sequences identified to date for a Brucella strain. In multilocus VNTR (variable-number tandem-repeat) analysis, strain BO1(T) showed a very distinctive profile and clustered with the other 'exotic' Brucella strains, including strains isolated from marine mammals, and Brucella microti, Brucella suis biovar 5 and Brucella neotomae. Biochemical profiling revealed a high degree of enzymic activity and metabolic capabilities. Incomplete lysis was detected with bacteriophages Tb (Tbilisi), F1 and F25. Strain BO1(T) reacted with Brucella M-monospecific antiserum. Strain BO1(T) harboured four to five copies of the Brucella-specific insertion element IS 711, displaying a unique banding pattern, and exhibited a unique 16S rRNA gene sequence and also grouped separately in multilocus sequence typing analysis. ![]() Affiliation of strain BO1(T) to the genus Brucella was confirmed by means of polyamine pattern, polar lipid profile, fatty acid profile, quinone system, DNA-DNA hybridization studies and by insertion sequence 711 (IS711)-specific PCR. piscicida, qui constitue la base d'un vaccin contre une infection à photobacterium, permettant de protéger des poissons d'une pasteurellose.A Gram-negative, non-motile, non-spore-forming coccoid bacterium (strain BO1(T)) was isolated recently from a breast implant infection of a 71-year-old female patient with clinical signs of brucellosis. Un dérivé d'une protéine extracellulaire de 55kDa issue de photobacterium damselae subsp. piscicida is the basis for a vaccine against photobacterium infection, and thereby protects fish from pasteurellosis. Il est indiqué dans le traitement du complexe respiratoire bovin chez les bovins de boucherie et de la pasteurellose chez les agneaux.Ī derivative of a 55kDa extracellular protein from photobacterium damselae subsp. Micotil est un médicament breveté pour usage vétérinaire produit par Eli Lilly et distribué au Canada par Elanco-Provel. Micotil is a proprietary medicine for veterinary use produced by Eli Lilly and distributed by Elanco-Provel in Canada and is indicated for the treatment of bovine respiratory complex in beef cattle and the treatment of pasteurellosis in lambs. Par exemple, en 2006, une population de bœufs musqués introduite en Norvège a été frappée par une flambée de pasteurellose qui a tué 20 % de la population. ![]() Théoriquement le diagnostic de pasteurellose est difficile sans l'aide du laboratoire car les formes respiratoires sont peu ou pas différentes de celles provoquées par d'autres germes et les formes abcèdatives peuvent être d'origine staphylococcique.įor example, an introduced muskox population in Norway was struck by an outbreak of pasteurellosis in 2006, killing about 20% of the population. ![]() Theoretically the diagnosis of pasteurellosis is difficult without laboratory help because respiratory forms are hardly or not different from those caused by other bacilli, and forms involving abscesses may have a staphylococcal origin. It must have a broad spectrum, covering the most common germs (eg pasteurellosis, staphylococci, streptococci, anaerobic).Įlle doit avoir un spectre large, couvrant les germes les plus fréquents (notamment pasteurellose, staphylocoques, streptocoques, anaérobies). Le producteur vigilant peut réduire les cas de pasteurellose dans son exploitation. Producers who are vigilant in their management of pasteurellosis can reduce its occurrence on their farms.
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